Actinobacillus actinomycetemcomitans의 Lipopolysaccharide에 대한 단클론항체의 제작 및 특성
Production and characterization of monoclonalantibody to lipopolysaccharide of Actinobacillus
actinomycetemcomitans
성진효, 이영종, 장기완, 이순례,
소속 상세정보
성진효 ( ) - 조선대학교 치과대학 예방치학교실,
이영종 ( ) - 광주보건대학 임상병리과
장기완 ( ) - 전북대학교 치과대학 예방치학교실
이순례 ( ) - 조선대학교 치과대학 예방치학교실
KMID : 0355420030270040611
Abstract
Actinobacillus actinomycetemcomitans(A. actinomycetemcomitans), a nonmotile, gram negative facultative anaerobic coccobacillus, is supposed to be an important etiological agent in localized juvenile periodontitis and associated with adult type periodontitis. Lipopolysaccharide (LPS) is one of the various virulent factors of A. actinomycetemcomitans and can directly or indirectly cause destruction of periodontal tissue. The purpose of this study was to produce and characterize the monoclonal antibody to LPS of A, actinomycetemcomitans.
The results were as follows;
1. The LPS yield of A. actinomycetemcomitans was 0.7% based on dry cell weight.
2. The antibody titers of immuninzed serum with 5.2 x 108 bacteria were 1:64,000 to whole cells and 1:8,000 to LPS.
3. From three times of cloning the antibody producing hybridomas using limitation dilution method, ten
monoclones producing monoclonal antibody to LPS of A. actinomycetemcomitans were obtained.
4. Ten monoclonal antibodies to LPS of A, actinomycetemcomitans were not found to cross-react with LPS
of Escherichia coil (E.. cob).
These results indicate that the monoclonal antibody to LPS of A. actinomycetemcomitans was specific for A. actinomycetemcomitans
키워드
단클론항체;Actinobacillus actinomycetemcomitans;Lipopolysaccharide(LPS);monoclonal antibody
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